General Information: Glutathione (GSH) is composed of three amino acids combined to produce a peptide that is both a powerful antioxidant and performs several critical roles in the body. According to researchers this peptide is so essential to optimum health that the level of Glutathione in cells could possibly be used to predict how long an organism lives.12
Glutathione catalyzes glutathione S-transferases (GST) and glutathione peroxidases (GPx). Thus, playing a role in detoxification by eliminating toxic electrophilic molecules and reactive peroxides. Glutathione plays a crucial role in a detoxification system that is fundamental in plants, mammals, and fungi.3
Aside from its detoxification role it is important for a variety of essential cellular reactions. Its presence in the glyoxalase system, is fundamental to DNA and RNA nucleotide reduction. Glutathione is also a constituent in the regulation of protein and gene expression, exchange reactions including thiol to disulfide ratios involve glutathione.4
Glutathione can exist intracellularly in either an oxidized (glutathione disulfide) or reduced (glutathione) molecular state. The ratio of reduced glutathione to glutathione disulfide has been shown to be critical in cell survival, this system is very tightly regulated.
Deficiency of glutathione puts the cell at risk for oxidative damage. An imbalance of glutathione is present in many pathologies including cancer, neurodegenerative disorders, cystic fibrosis (CF), HIV and aging.
While Glutathione is vitally essential to maintaining a healthy immune system, it isn’t classified as an essential nutrient; this is because the body does create its own supply from the amino acids:
- L-glutamic acid
One of the reasons why Glutathione is so important for optimum health is that it’s present in every cell in the body. One way antioxidants like glutathione help maintain good physical health is by neutralizing free radicals, which can cause cellular damage through oxidation. Since glutathione is naturally present within all types of cells, it is in a prime position to do this. It’s considered one of the most important antioxidants in the human body.5
Mechanism of Action: Glutathione is an essential molecule required for detoxification. Glutathione acts by assisting the body’s machinery in the removal of harmful destructive oxygen containing molecules.
During the body’s normal functioning an excess of oxygen containing molecules are produced, these molecules are typically very reactive with other molecules they come in contact with. In modern biochemistry these are referred to as reactive O2 species.
Reactive O2 species molecules include peroxide (H2O2) and superoxide anions (O2 with unpaired electron) these molecules are very toxic to the cell. The toxicity can be explained by the tendency of these molecules to bind or destroy important biomolecules.
The body has a natural system to remove these reactive O2 species. These systems metabolize and scavenge for reactive oxygen species, in a controlled and precise fashion.
The system that removes these toxic reactive oxygen species includes a host of enzymes including four enzymes
- Glutathione peroxidase (GPX)- GPX detoxifies peroxides with glutathione acting as an electron donor in the reduction reaction, producing glutathione disulfide as an end product. GPX is a 80 kDa protein that is composed of four identical subunits. It is expressed throughout the entire body, individual isoforms are present in specific tissues. When the body is in a state of excess oxidative stress the expression of this enzyme is induced. Abnormal expression has been associated with a wide variety of pathologies, including hepatitis,6 HIV,7 and a wide variety of cancers, including skin,8 kidney,9 bowel,10 and breast.11
- Glutathione reductase (GR)- catalyzes reduction of glutathione disulfide is by requires NADPH producing two glutathione molecules as an end product. GR is a member of the flavoprotein disulfide oxidoreductase family and exists as a dimer.4 Expression of GR is upregulated during periods of increased oxidative stress, to prepare for reactive oxygen species removal. The level at which regulation takes place is at the transcriptional level as well as at the post-translational level. Down regulation of GR production and activity are thought to be associated with cancer and aging.12
- Catalase- is involved in detoxification of reactive oxygen species.
- Superoxide dismutase (SOD)- is involved in the removal of superoxide species.
Glutathione and immune function: Glutathione plays a significant role in immune function.13 It encourages the T-cell function that’s essential for a healthy immune system14 and protects from environmental toxins.
- Glutathione acts to neutralize a toxic metabolic byproduct: Methylglyoxal
- Glutathione is involved in the protein disulfide bond rearrangement that is necessary for the synthesis of one third of the body’s proteins
- It protects the body from the oxidative damage caused by glutathione peroxidase by acting as a helper molecule for certain enzymes
- The liver uses Glutathione to help detoxify fats before the gallbladder emits bile, supporting healthy digestion
Glutathione to remove and detoxify carcinogens: Glutathione may also be crucial in the removal and detoxification of carcinogens, and according to recent studies17 alterations in this metabolic pathway, can influence cell survival profoundly. Glutathione may be responsible for several vital roles within a cell besides antioxidation: 17
- Maintenance of the redox state (chemical reactions in which the oxidation state of atoms are modified)
- Modulation of the immune response
- Detoxification of foreign bacteria and viruses
Glutathione and chronic disease: Research has demonstrated that glutathione deficiency may be a factor in many chronic conditions; HIV/AIDS, Alzheimer’s, Parkinson’s disease, asthma, different cancers, cataracts, macular degeneration, open angle glaucoma, diabetes, and many diseases of the liver, kidneys, lungs, and digestive system.18
Glutathione depletion due to aging and alcohol consumption: Glutathione plays a major role in the detoxification of ethanol (consumed as alcoholic beverages) and people who routinely drink will experience Glutathione depletion.19 Aging is another factor; as the body ages glutathione levels may drop below the level necessary to maintain healthy immune function (among other processes).19
- Benzopyrenes (tobacco smoke, fuel exhaust, etc.)
- Many household chemicals (detergents, fabric softeners, air fresheners, mothballs, cleaners, bleach, etc.)
Glutathione for male fertility: In a study of eleven infertile men, suffering from dyspermia associated with various andrological pathologies - Glutathione was observed to exert a significant effect on sperm motility. Glutathione appeared to have an observable therapeutic effect on certain andrological pathologies that cause male infertility.24
Glutathione and artherosclerosis: In one study, ten patients with artherosclerosis were administered glutathione which resulted in a significant increase in blood filtration, in addition to a significant decrease in blood viscosity and platelet aggregation. Consequently, Glutathione infusion was determined to be an effective method of decreasing blood viscosity while increasing blood filtration.25
Glutathione has dermatological properties: In a three-month study of female subjects, the women taking Glutathione showed significantly improved skin elasticity and amelioration of wrinkles compared to test subjects who received a placebo.26
- Stimulate the immune function
- Improve skin properties
- Improve semen parameters
- Decrease blood viscosity
- Increase lower than optimal levels of glutathione in the body due to aging and environmental stressors
What are the precautions when taking this medicine? Nitric oxide could react with glutathione to create s-nitrosoglutathione, a molecule that can act as a vasodilator. Vasodilation can lead to lowered blood pressure. This side effect may not be safe for people who have hypotension.32
What are some possible side effects of this medicine? Studies haven’t shown any side effects when supplementing with Glutathione.
How is it best taken? Glutathione can be taken either orally, with a transdermal patch or by intravenous/subcutaneous/intramuscular injection. Studies show that the most effective way to increase glutathione levels is by injection.33
What do I do if I miss a dose? If you do miss a dose; it’s best to take it as soon as you remember. Although, if it’s almost time for the next dose, just skip the missed one and take your next scheduled dose. Don’t take two doses at the same time.
How should I store this medicine? Store Glutathione at between 32°F to 38°F (2°C to 8°C). Keep all medicines out of the reach of children. Throw away any unused medicine after the expiration date. Do not flush unused medications or pour down a sink or drain.
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